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Zaiton, A.*; 大野 豊
JAEA-Review 2015-037, 120 Pages, 2016/03
JAEA-Review-2015-037.pdf:15.19MB
本報告書は、「放射線加工処理の分野における研究協力に関するマレーシア政府と日本原子力研究開発機構との間の実施取決め」に基づいて2002年12月から2012年12月にかけて国立研究開発法人日本原子力研究開発機構とマレーシア政府下の研究実施機関であるマレーシア原子力庁により実施された二国間研究協力についてまとめたものである。この間に実施された2つのイオンビーム育種分野における研究プログラム「イオンビームによるランの突然変異誘発」(2002年12月
2007年12月)及び「イオンビームによる新規観賞植物品種の作出」(2007年12月2012年12月)の研究活動に加え、付録としてそれぞれの研究プログラムに係わる運営員会の開催状況、イオンビーム照射実験の実施状況、論文・口頭発表リストについて収録した。
pink mutants through ion beam irradiationZaiton, A.*; Affrida, A. H.*; Shakinah, S.*; Nurul Hidayah, M.*; 長谷 純宏; 大野 豊
JAEA-Review 2014-050, JAEA Takasaki Annual Report 2013, P. 108, 2015/03
Developing new varieties of chrysanthemums is one of the emphases in Malaysian floriculture research as chrysanthemum is recognized as among the most important cut flower export for Malaysia. A combination of ion beam irradiation and 
organ culture techniques is very efficient in generating new ornamental varieties with novel traits and wider mutation spectrum. Therefore, in this project, ray floret cultures of pink chrysanthemum variety were irradiated at various doses with 320 MeV 
C
ions, regenerated into plantlets and screened for morphological variations with an ultimate aim to produce mutants with useful horticultural traits. A series of screening was carried out at Nuclear Malaysia's glasshouse and MARDI Cameron Highlands. The mutants were selected and characterized morphologically. Based on these screenings, three mutants were identified based on their distinct flower and/or plant characteristics.
Zaiton, A.*; Shakinah, S.*; Affrida, A. H.*; Nur Akmal, M. R.*; Siti Mardhiah, M.*; 大野 豊
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In this study, the effects of ion beams on chrysanthemum flower colour traits of were examined through morphological and molecular analysis. Ray floret and nodal cultures of 
cv. Reagan Red were irradiated at different doses using ion beams irradiation facility at Japan Atomic Energy Agency (JAEA), Takasaki, Japan. Morphological screening on the regenerated mutant flowers has shown that 14 different primary colour types based on The Royal Horticulture Society Colour Chart were observed. Majority of the plants produced flowers with intense or lighter colour in the red group, and a few with orange and greyed-purple colour. Molecular analysis involving three genes in flavonoid biosynthesis pathway (CHS, DFR and F3H) showed that intragenic mutation involving base substitution, deletion and insertion has occurred in mutant plants. The highest mutation was observed in F3H, followed by CHS and DFR fragments with 20.5, 17.2 and 5.5% base mutation respectively.
